Relationship between DNA methylation in the 5’ CpG island of the SLC47A1 (MATE1) gene and inter-individual variability in MATE1 expression in the human liver
نویسندگان
چکیده
Fig. S1 CRISPR/Cas9-mediated genome editing in HepG2 Cells A) top, schematic depiction of CRISPR-targeted CpG island sequence. CRISPR guide sequences (gRNA-1 and gRNA-2) are highlighted in grey, and proto-spacer adjacent motif (PAM)s are underlined. A) bottom, the target region was eliminated in nonhomologous end joining DNA repair. Primer pairs used to determine the target deletion are indicated. B) Target deletion was determined by PCR using primer pair outside the deleted region. Successful deletions of the target region were observed in clone No.4 to 6. NT, non-treated HepG2 cells, DNA ladder, φX174 HaeIII digest. A
منابع مشابه
Relationship between DNA Methylation in the 5' CpG Island of the SLC47A1 (Multidrug and Toxin Extrusion Protein MATE1) Gene and Interindividual Variability in MATE1 Expression in the Human Liver.
Multidrug and toxin extrusion protein 1 (MATE1), which is encoded by solute carrier 47A1 (SLC47A1), mediates the excretion of organic cations into bile and urine. Some genetic variants in human MATE1 altered its transport function in in vitro experiments; however, differences in the pharmacokinetics of substrate drugs cannot be explained by genetic variations in humans. In this study, we invest...
متن کاملStudy of promoter CpG island hypermethylation of cyclindependent kinase inhibitor gene p21waf1/cip1 on some breast carcinoma cell lines
The p21 belongs to the CIP/KIP family of CDK inhibitors involved in cell cycle arrest at specific stages of the cell cycle progression. DNA methylation is the best studied epigenetic mark that have been evidently associated to chromatin condensation, and repression of gene transcription. The CpG island hypermethylation in promoter region of certain genes occurs in cancer cells and affects tumor...
متن کاملTargeted disruption of the multidrug and toxin extrusion 1 (mate1) gene in mice reduces renal secretion of metformin.
Multidrug and toxin extrusion 1 (MATE1/SLC47A1) is important for excretion of organic cations in the kidney and liver, where it is located on the luminal side. Although its functional and regulatory characteristics have been clarified, its pharmacokinetic roles in vivo have yet to be elucidated. In the present study, to clarify the relevance of MATE1 in vivo, targeted disruption of the murine M...
متن کاملSignificance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury
BACKGROUND/AIM To elucidate the mechanism responsible for developing acute kidney injury in patients with diabetes mellitus, we also evaluated the issue of whether advanced glycation endproducts (AGEs) influence the expressions of multi antimicrobial extrusion protein (MATE1/SLC47A1) in tubular cells. MATERIALS AND METHODS To detect changing expression of MATE1/SLC47A1 in dose- and time-depen...
متن کاملHuman multidrug and toxin extrusion 1 (MATE1/SLC47A1) transporter: functional characterization, interaction with OCT2 (SLC22A2), and single nucleotide polymorphisms.
Renal elimination of a number of cationic compounds is thought to be mediated by the organic cation transporter 2 (OCT2, SLC22A2), a drug uptake transporter expressed at the basolateral domain of renal tubular cells. Recently, the key efflux transporter for the secretion organic cations was identified as an electroneutral H(+)/organic cation exchanger termed the multidrug and toxin extrusion (M...
متن کامل